Interaction of Dichloro (2-aminoethylsulfonyl) platinum (II) Complex with DNA Constituent Nucleosides

Abstract

Cis-diamminedichloroplatinum(ll) complex (cis-DDP) is widely utilized as an anticancer agent for clinical treatments of various cancers. It has been confirmed by many evidences that cis-DDP binds specifically with DNA in cancer cells to inhibit the replication of the DNA. Therefore, many investigations concerning novel platinum complexes with various carrier ligands have been developed to be used as anticancer agents. However, the study on the platinum complex coordinated with a ligand containing sulfur atoms has not been reported yet. In this paper, a novel platinum complex, dichloro(2-aminoethylsulfony1)platinum(II), was synthesized. The structure of the platinum complex was analyzed by IR and ¹³C-NMR spectra, elementary analysis and atomic absorption spectrophotometry. The investigations were also carried out on the interaction between the platinum complex and DNA constituent nucleosides by analyzing the UV difference spectra and the liberation rate of the chloride ions from the platinum complex. The isosbestic points were observed in the UV difference spectra for the platinum complex-nucleoside system. It was also found that two chloride ions were liberated from the platinum complex in the interaction with the nucleosides. The platinum complex was found to exhibit a specific cytostaticcytotoxic activity against adriamycin resistant cancer cells.