Binding of Proteins onto Highly Porous Resin Beads Modified with Isothiocyanato Groups

Abstract

Highly porous poly(styrene-co-divinylbenzene) resins were coupled with 3-chloropropionyl chloride by Friedel-Crafts reaction. The resins obtained were reacted with KSCN in DMF solution at 80°C to introduce isothiocyanato groups. The resin beads were examined for the binding ability with proteins by using batch method and column injection method. Proteins were attracted to the resin beads by the hydrophobic interaction. Subsequently proteins were strongly bound to the resin beads by formation of a stable thiourea structure between amino groups in the proteihs and isothiocyanato groups on the resin beads. These resin beads packed in a column were useful for the pretreatment (removal of proteins) of serum samples in the continuous quantitative analysis of glucose using immobilized enzymes. These resin beads were also useful as the supports of immobilized enzymes.