Flame Photometric Detector of. Micro Liquid Chromatography by Means of Electrospray of Eluent

Abstract

A flame photometric detector (FPD) for micro liquid chromatography has been developed, where the eluent was introduced into FPD by an electrospray technique. This method is useful to detect such agricultural chemicals as contain sulfur and do not have UV absorbance. The quenching due to organic solvent was decreased by using such double flame burner similar to be used in gas chromatography.1-Propanol and 2-propanol used as eluent were nebulized and burned stably in hydrogen-air flame, but not for less polar liquids such as benzene and toluene by the electrospray method. On the other hand, 40% (v/v) 2-propanol/water did not burned stably in hydrogen-air flame, but burned stably in hydrogen-oxygen flame. The chromatograms of four alkyl phenylthiocarbamates were obtained with the ODS column (0.5 mm in i. d. and 110 mm in length, eluent: 40% (v/v) 2-propanol/water, flow rate: 8 μl/min) coupled with FPD detection. The optimum working conditions of FPD were as follows: flow rate of hydrogen for lower flame and upper flame were 72 ml/min and 109milmin, respectively, and flow rate of oxygen was 60 m//mm. The detection limit of hexan al thiosemicarbazone was 0.2 μg.