Formation of Inulo-oligosaccharides by β-Fructofuranosidase from Aspergillus Oryzae

Abstract

Inulo-oligosaccharides( I ) production, by use of immobilized p-fructofuranosidase from Aspergillus Oryzae was investigated. Chito-pearl-BCW-4010 was the best carrier for immobilization of the enzyme. ' The optimum temperature was 25°C and the optimum pH was 6.8 for production of ( I ) from sucrose and fructose. When the molar fraction of sucrose was increased for sake of forming of enzyme-fructose complex, sucrose acted as an acceptor sugar to form fructooligosaccharides. This dose not contribute to formation of ( I ). A disubtituted sucrose derivative retaining (Fruf 2 β--α 1 Glcp) bond reacted with immobilized enzyme to form the complex. This complex reacted with fructose to form the disubtituted inulobiose which was converted to inulobiose in good yield.