Trace Analysis of Phospholipids by Enzymatic Reactions on a Silica-Gel Plate

Abstract

The purpose of the present study was to conduct a basic work of the tr ace analysis of phospholipids in biological urine and serum samples. Phospholipids were extracted with chloroform using Extrelut column and developed by TLC. Solutions of PL-C and Al-P were sprayed on the TLC plate (silica gel), and released phosphate was detected with molybdate-Malachite Green solution. This method can be easily used for the separation and determination of phospholipid content as low as pmol.