Specific and Highly Sensitive Quantitative Analysis for Proteins by the Combination of Affinity Chromatography and Colloidal Platinum Stain i n g Method

Abstract

A specific and highly sensitive detection method for protein was developed. The method is the combination of protein separation with affinity chromatography and the staining method by colloidal platinum. By using this method human serum albumin and human insulin were detected with high sensitivity such as 1 ng.