Hydrolysis of Beef Tallow by Immobilized Lipase in a Biphasic Organic-Aqueous System

Abstract

Lipase from Candida cylindracea was immobilized on chitosan beads by covalent binding methods, and its application to hydrolysis of beef tallow was investigated in a biphasic organic-aqueous system. The addition of isooctane, found to be the best among the organic solvents tested, to the reaction system stimulated the hydrolysis of beef tallow by immobilized lipase strongly. The proportion of isooctane and aqueous buffer in the reaction mixture was chosen at the ratio of 1: 4. Under this condition, the reaction rate was greatly enhanced compared to that in the organic-solvent-free system, and the extent of hydrolysis reached to about 90%.