Size-Exclusion Chromatography (SEC) of High Molecular Weight DNAs

Abstract

Size-exclusion chromatography (SEC) of DNAs was investigated with stainless steel columns (6 mml. D. x 250 mm) packed with macroporous spherical gels prepared from glucomannan. High molecular weight DNAs (>15 kb) were eluted following size-exclusion theory from the columns packed with large gels (>130 earn). However, the smaller gels resulted in the larger elution times for those DNAs. The particle size as well as the pore size of the packing material were found to be important factors for chromatographic separation of high molecular weight DNAs.