Development of Conductometric Enzyme Sensors Using Microarray Electrodes

Abstract

This paper describes the sensing characteristics of conductive polymer/enzyme-coated microarray electrodes consisting of assembly of microband electrodes with a few μ m width and a few mm length. The sensing principle of the array devices is based on detection of conductometric changes induced by chemical reactions of analytes. The followings are the contents of this paper: 1. Fabrication of ultrathin conductive polymers at hydrophobically-pretreated microarray electrodes. 2. n situ conductivity measurements using microarray electrodes coated with conductive polymers. 3. Redox behavior of polypyrrole-coated microarray electrodes. 4. NADH switching behavior of polypyrrole/diaphorase-coated microarray electrodes. 5. pH sensing behavior of polypyrrole- or polyaniline-coated microarray electrodes. 6. Penicillin sensing behavior of polypyrrole/penicillinase -coated microarray electrodes. 7. Glucose sensing behavior of polyaniline/glucose oxidase-coated microarray electrodes.