Abstract
Background A chemiluminescence enzyme immunoassay (CLEIA) for high-molecular-weight-adiponectin (HMW-ADPN) was recently developed, and has been shown to be faster, more accurate and less expensive than conventional ELISA. In the present study, we investigated relationships between metabolic parameters and HMW-ADPN levels measured by CLEIA in the health check-up setting.
Methods Informed consent was obtained from 1,036 males and 416 females who participated in this study, comprising Japanese teachers and other working individuals ranging from 40 to 71 years in age. Subjects receiving anti-diabetic medication or with suspected inflammation [C-reactive protein (CRP) level > 1 mg/dL] were excluded from the analyses.
Results Median levels of serum HMW-ADPN measured by CLEIA were 2.50 μg/mL in males and 4.95 μg/mL in females. The serum HMW-ADPN level in males with metabolic syndrome (MetS) (2.13±1.14, n = 173) was significantly lower than that in those without MetS (3.02 ± 3.43, n = 860, P < 0.0001). HMW-ADPN levels were correlated positively with serum high-density lipoprotein (HDL)-cholesterol in both males and females, and correlated inversely with body mass index, waist circumference, systolic blood pressure, plasma glucose, serum triglycerides, uric acid, CRP, insulin resistance index (HOMA-IR), etc. Stepwise multiple regression analysis revealed that the serum HMW-ADPN level was correlated independently with serum HDL-cholesterol, sex, HOMA-IR and CRP (F < 20, P < 0.0001, R2 = 0.392).
Conclusions These results suggest that measuring serum HMW-ADPN by CLEIA is useful, and that the serum HMW-ADPN level is closely correlated with parameters related to MetS and/or cardiovascular diseases.
