Abstract
Eight glucocorticoids (GCs) were added to logarithmically growing cultures of primary human skin fibroblasts. All GCs tested inhibited DNA synthesis of the human fibroblasts, at concentrations of 0.1, 1, 10 μg/ml. From the dose-response curves of three different concentrations of GCs on the 3H-uptake in the cells, we estimated the GC concentrations causing a 50% inhibition of DNA synthesis. The order of the concentrations of each GC seems to correlate with their anti-inflammatory potency as evaluated clinically. We wish to emphasize that these doses are good indices for not only testing the anti-proliferative and anti-inflammatory potencies of GCs but also for their atrophogenic property, as related to skin.
