2002 Volume 51 Issue 4 Pages 779-781
The protein product of the c-Cb1 proto-oncogene is involved in several signaling pathways and exerts a negative regulatory function on receptor and non-receptor tyrosine kinase. We used the overexpression experiment, and demonstrated that the c-Cb1 inhibits phosphorylation of intracellular protein in osteosarcoma cell line, SaOS2. Luciferase reporter assays revealed overexpression of c-Cb1 suppressed PDGF-induced c-Fos promoter activity as well as v-Src-induced STAT3 promoter activity. Thus, our results demonstrate that c-Cb1 negatively regulates several signaling pathways in the osteosarcoma cell line, suggesting that c-Cb1 is a good candidate for gene therapy against osteosarcoma.
