Abstract
This report concerns itself with the bacteriologic study on bile in the gall bladderand the common bile duct, the wall of the gall bladder and lymph glands at neck ofthe gall bladder in a total of fifty two patients who had cholecystectomy for biliarypassage diseases.
The sensitivity of the isolated bacteria such as E. coli, Micrococci, B. proteus and Staphylococci to various antibiotics was investigated.
Thirty strains of Staphylococci isolated from various purulent lesions other than thebiliary passage were also studied on their sensitivity to antibiotics.
Results of the investigations are as follow:
1) The frequency, of isolation of microorganism from the wall of gall bladder, lymphglands at neck of gall bladder and bile in common bile duct in their smears was halfas frequent as in cultures of the same materials.
2) The frequency of isolation of organisms from the wall of gall bladder was 47 outof 52 (90.3%), 26 out of 31 (83.8%) from the lymph glands, 39 out of 52 (75.0%) from bilein ductus choledocus and 32 out of 52 (61.5%) from bile in gall bladder. The frequencyof isolation of organisms was higher in the culture of emulsion of tissues than bile.
3) E. coli was most frequently encountered among a total of 155 isolated strains.There were 66 strains of E. coli (42.6%), 39 strains of Staphylococci (25.2%) 19 strainsfo Micrococci (12.3%), 14 strains of B. proteus (9.0%) and 8 miscellaneous strains.
12 strains out of 19 isolated Micrococci were Urease-producing. Since there were 14Urease-producing B. proteus, the total number of isolated Urease-producing microorganismsamounted to 26 (17.6%) strains.
Coagulase test on Staphylococci reviealed “Positive” in 30 strains out of 39.
4) Sensitivity tests revealed that over 40% of all isolated organisms but Micrococciwere found to be resistant to Penicillin. B. proteus was sensitive to Streptomycin andothers were fairly sensitive to it.
As to the sensitivity test on Sulfa drug, Micrococci were somewhat sensitive, butresistance to the drug was found in 60 or 70% of other organisms.
Chloramphenicol, Erythromycin and Tetracyclin had comparatively good antibacterialaction. There was found non-sensitive strain in only 10 or 15% of all isolated organisms.
