[title in Japanese]
1960 Volume 57 Issue 1 Pages 51-70_2
Details
1960 Volume 57 Issue 1 Pages 51-70_2
The changes of the chemical composition of liver tissue, obtained by needle biopsy in health and diseases, were estimated by ultra-micromethod.Furthermore, experimental studies were made on the rat liver feeded by dried cells of Penicillium islandicum Sopp and toxic rice containing the fungi for comparing with the clinical studies.
The results obtained were as follows.
1) As the method of chemical determination of collagen, the condition of Gordon Fel's method determining hydroxyproline was investigated and it was found that this method was quite satisfactory.
2) The ultra-micromethod using few milligrame of liver tissue obtained by biopsy was observed to be significant for the analysis of chemical composition.
3) In acute liver injury, the changes of enzyme activities were remarkably noted clinically and experimentally.However, the chemical composition did not show any change except the decrease of liver glycogen.
4) In cases of chronic liver diseases any chemical change was not observed.In rat liver, the amount of collagen increased when fibrosis was found histologically.
5) In experimental cirrhotic livers, fibrosis and increase of collagen were remarkable. The content of collagen increased from 5 to 7 folds compared with the normal level. The increase of hexosamine was noted in fibrous part of liver.
6) It was concluded that the experimental liver cirrhosis by P.ial.Sopp was a postnecrotic form, not developed via fatty liver, since the fatty infiltration was not found chemically and histologically these animals.
7) The decreases of liver protein, serum protein and serum albumin were observed in experimental liver cirrhosis.The serum γ-globulin increased and the content of hemoglobin decreased.8) The increase of collagen of various liver diseases was chemically estimated.The highest level was obesrved in cholangioma and lowest was in Banti's syndrome.The increase of collagen was moderate in Wilson's disease, Pick's disease, Laennec's liver cirrhosis, acute yellow liver atrophy, biliary liver cirrhosis and hepatoma.
