Studies on the excretory function of the liver

Influence of liver hydrolysate upon the biliary excretion of sulfobromophthalein

Abstract

Rat experiments were undertaken to examine the alterations of steps involved in the-transfer of BSP from blood to bile following the intravenous administration of liver hydrolysate (Proheparum, Nordmark-Kaken).
1) Biliary excretion of BSP was increased by the intravenous administration of liver hydrolysate.
2) Through the administration of liver hydrolysate, a significant increase of unconjugated BSP storage in the liver and a moderate degree of cholereis were demonstrated. No acceleration of BSP-gultathione conjugating enzyme activity, elevation of biliary BSP-Tm, inhibitionof BSP-binding affinity of serum albumin and release of BSP from BSP-bound serum were observed.
3) The above facts suggest that the most important factor to increase biliary excretion of BSP following the intravenous administration of liver hydrolysate may be an increased hepatic storage of BSP, namely, an increased permeability of hepatic cell membrane especially on the uptake side from the blood stream.