Occurrence of Variant of Hepatic Alkaline Phosphatase Isoenzyme in a Case of Acute Viral Hepatitis

Abstract

Isoenzymes of alkaline phosphatase (ALP) are usually normal in the serum of acute viral hepatitis. We discovered a case of acute hepatitis who had an abnormal ALP isoenzyme in blood. This isoenzyme migrated more toward cathode than intestinal ALP by electrophoretic separation of isoenzymes. Enzymologic natures of this isoenzyme were similar to those of normal hepatic isoenzyme on inhibition by L-phenylalanine, L-homoarginine and L-leucine. This isoenzyme was detected in the liver extract also. Decrease in electrophoretic mobilities ofisoenzymes was seen both in hepatic and abnormal ALP by neuraminidase digestion, but retardation rate was more scarce in abnormal ALP. These data indicate that this isoenzyme is made by the liver which exhibits abnormal metabolism.