A PATHOPHYSIOLOGICAL STUDY OF PHOSPHATE IN EXPERIMENTAL CELL DESTRUCTION OF PANCREAS AND ITS APPLICATION TO CLINICAL DIAGNOSIS

Abstract

By using the homogenate of dog pancreas, catabolism of phosphate was studied in the destructed tissue. Phosphate was very high in concentration in the pancreas tissue. The fraction of acid insoluble phosphate consisted of nucleic acid-P and lipid-P, and these values were 201.1μg (54.9%) and 61.8μg (16.9%) per 100mg of pancreas tissue respectively. Acid insoluble phosphate was changed into acid soluble phosphate, finaly into inorganic phosphorus (H₂PO^-₄), in 24-hour incubation at 37°C.
The amounts of inorganic phosphorus of the duodenal juice collected at performance of pancreozymin-secretin (PS) test was ordinaly after 24 hour incubation at 37°C, and its value of the secretin phase was 10.0±3.7μg P/ml in normal subjects (n=35). In the patient suspected to be chronic pancreatitis, the value of inorganic phosphorus of the duodenal juice was compared with three factors of PS test (volume, maximum bicarbonate and amylase output) and the findings of endoscopic retrograde pancreatography (ERP). Abnormal findings of ERP were frequently observed even in the patient with normal PS test. On the contrary, the patient with abnormal ERP findings was abnormal in concentration of inorganic phosphorus of the duodenal juice.
However, inorganic phosphorus increasing in the duodenal juice was thought to originate from the contamination with bile and/or mucosal juice, and so it was necessary to investigate pure pancreatic juice.