1980 Volume 77 Issue 4 Pages 572-582
The purpose of this study is to assess the re of microtubules in the process of absorption and transportation of long chain fatty acids into intestinal lymph and to clarify whether intestinal alkaline phosphatase (IAP) plays a part in the phase of lipoprotein release to lymphatics during fat absorption.
Male Wistar rats provided with intestinal lymph fistula were used for the absorption studies. Mixed micellar solutions of fatty acids were administered into the duodenum of rats. Effect of colchicine, an inhibitor of microtubular function, on the fatty acid absorption and alkaline phosphatase activity during fat absorption were studied in intestinal lymph.
In colchicine-treated rats, the lymphatic absorption of intraduodenally administered long chain fatty acids was decreased, however, there was no significant difference of colchicine inhibition between the lymphatic absorption of palmitic acid (saturated) and linoleic acid (unsaturated). Administered fatty acid was transported slowly to lymphatics almost in form of free fatty acid when colchicine was treated. Microtubular system was revealed to be necessary for the lipoprotein release from intestinal epithelial cells to lymphatics during fatty acid absorption.
In control rats, the increased activity of TAP in intestinal lymph was observed in parallel with the amount of absorbed fat. However, in colchicine-treated rats, the equal amount of output of TAP to lymphatics was also observed in spite of the decrease of linoleic acid absorption. These results suggest that microtubular system does not take part in the transportation of TAP into lymphatics and TAP may not play a possible role in the phase of secretion of lipoprotein from intestinal epithelial cells during the absorption of long chain fatty acid.
Nippon Shokakibyo Gakkai Zasshi
Nippon Shokakibyo Gakkai Zasshi Kou
