等電点カラム電気泳動法による催胆汁うつ滞因子の分離精製

溝口 靖紘; 筒井 ひろ子; 阪上 吉秀; 志波 孝; 東森 俊博; 中尾 昌弘; 門奈 丈之; 山本 祐夫; 森沢 成司

doi:10.11405/nisshoshi1964.79.947

PURIFICATION OF THE CHOLESTATIC FACTOR PRODUCED FROM ACTIVATED GUINEA PIG LYMPHOCYTES BY MEANS OF ISOELECTRIC FOCUSSING ELECTROPHORESIS

Yasuhiro MIZOGUCHI, Hiroko TSUTSUI, Hideyoshi SAKAGAMI, Takashi SHIBA, Toshihiro HIGASHIMORI, Masahiro NAKAO, Takeyuki MONNA, Sukeo YAMAMOTO, Seiji MORISAWA

Author information

Yasuhiro MIZOGUCHI
The Third Department of Internal Medicine Osaka City University Medical School
Hiroko TSUTSUI
The Third Department of Internal Medicine Osaka City University Medical School
Hideyoshi SAKAGAMI
The Third Department of Internal Medicine Osaka City University Medical School
Takashi SHIBA
The Third Department of Internal Medicine Osaka City University Medical School
Toshihiro HIGASHIMORI
The Third Department of Internal Medicine Osaka City University Medical School
Masahiro NAKAO
The Third Department of Internal Medicine Osaka City University Medical School
Takeyuki MONNA
The Third Department of Internal Medicine Osaka City University Medical School
Sukeo YAMAMOTO
The Third Department of Internal Medicine Osaka City University Medical School
Seiji MORISAWA
The First Department of Biochemistry, Osaka City University Medical School

JOURNAL FREE ACCESS

1982 Volume 79 Issue 4 Pages 947-951

DOI https://doi.org/10.11405/nisshoshi1964.79.947

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Abstract

The cholestatic factor showing a potent intrahepatic cholestatic activity was produced from the activated lymphocytes of tuberculine-snsitized guinea pigs by stimulation with PPD. The activity was observed in two fractions obtained by gel filtration on Sephadex G-75 followed by DEAE-cellulose column chromatography. When these two fractions were analyzed by means of an isoelectric focussing electrophoresis, it was shown that they contained different electric charges; one with an isoelectric point at 3.0-3.5 and the other at pH 7.8-8.0.

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