Analysis of antigens defined by anti-Anisakis larvae antibodies of IgE and IgG type in the sera of patients with acute gastrointestinal anisakiasis

Abstract

Using SDS-PAGE and Immuno-blotting assay, we analysed antigens defined by anti-Anisakis Larvae antibodies of IgE and IgG type in the sera of patients with acute gastrointestinal anisakiasis.
1) Humoral IgE response to 76, 64 and 60 kDa molecular weight proteins of Anisakis Larvae and IgG response to 91, 76, 64 and 60 kDa molecular weight proteins were demonstrated in the sera of patients with acute gastric anisakiasis. Those humoral antibody responses were also demonstrated in the sera of two patients with intestinal anisakiasis.
2) Those antibodies to 76, 64 and 60 kDa proteins may be Anisakis-specific antibodies, because anti-Anisakis Larvae antibodies of IgE and IgG type did not react with the similar MW proteins of Ascaris suum homogenates.
3) Also anti-Anisakis Larvae antibodies reacted with Anisakis Excretory and Secretory (E.S.) protein which included specific 76 and 60 kDa proteins, but they did not react with purified Anisakis hemoglobin.