Abstract
Axons are computational units in neuronal networks. Little is known about relationships between firing frequency and the extent of changes in axonal conduction velocity. Therefore, we stimulated axons extending through microtunnels with various stimulation frequencies, and recorded axonal conduction delays. Additionally, ZD7288, an h-current blocker, was added during stimulation to elucidate the mechanism underlying the change. Changes observed in the first 75 stimuli with a frequency of 20 Hz were smaller than those observed with lower frequencies, a tendency that was abolished by ZD7288. These results suggest that our method is capable of detecting activity-dependent changes in axonal conduction property.
