2-deoxy-D-glucose Inhibits Cell Activity by Inhibiting Intracellular Glucose Metabolism in Human Gingival Fibroblasts

Abstract

 Purpose: Cellular energy metabolism by glucose plays an essential role in tissue wound healing. The use of 2-deoxy-D-glucose (2-DG) to suppress energy metabolism through the glycolytic system is similar to a low glucose environment. Therefore, the purpose of this study was to investigate the effect of 2-DG-induced reduction in glucose metabolism on cell viability.

 Methods: We examined the proliferation, migration, cytotoxicity, H₂O₂ production, glucose transporter type 1 expression, lactic acid production, and ATP synthesis after the intracellular uptake of three different concentrations of 2-DG into HGnFs.

 Results: 2-DG suppressed intracellular glucose metabolism, which led to a reduction in the cellular activity of human gingival fibroblasts (HGnFs). However, there were no changes in cytotoxicity or H₂O₂ production, and the effects on glucose metabolism were different from those in a low glucose environment.

 Conclusion: This study showed that the suppression of intracellular glucose metabolism in HGnFs by 2-DG may delay wound healing in periodontal tissues.