Osteogenesis Around Dental Implants Combined with Cultured Rat Bone Marrow Cells

Abstract

In the present study, rat bone marrow (RBM) cells were cultured on dental implants. After primary culture, RBM cells were cultured on dental implants for 2 weeks with and without osteogenic factors. DNA, alkaline phosphatase activity, osteocalcin and osteopontin were measured. Biochemical analyses revealed that, on the dental implants, RBM cell differentiation into osteoblasts was induced by osteogenic factors. Dental implants with and without cultured RBM cells were implanted in the subcutaneous tissue or on the femur in syngeneic rats. Osteogenesis in the subcutaneous tissue and more extensive and faster bone formation around the femur were observed for the implants combined with cells cultured with osteogenic factors. These results demonstrated that dental implants combined with osteoblasts differentiated from RBM cells with osteogenic factors were effective for osseointegration and bone formation.