Abstract
Multiple myeloma (MM) is a plasma cell malignancy. Low proliferative activity of myeloma cells makes it difficult to perform the cytogenetic analyses. By comparative genomic hybridization (CGH), we detected a large number of chromosomal gains and losses in 3 sister myeloma cell lines (CLs), KMS-12BM/PE pair, KMS-21BM/PE pair, and KMS-28BM/PE pair, that were established from bone marrow (BM) and pleural effusion (PE) cells obtained from the same patient. The most recurrent gains were 1q, 11q and 18q, whereas the most recurrent losses were 9p, 16q, 17p and 22q. The smallest region of overlap (SRO) among the BM-origin CLs and the PE-origin CLs was identified. In the SRO, we deduced a candidate gene, such as IL6R, cyclin D1, BCL2, B7H1, p53, IRS 1, c-MYC, TNFSF8, and cadherin family genes, the amplification or deletion of which might be associated with disease progression, cell proliferation, transmigration and/or infiltration.
