Abstract
Subcutaneous adipose tissue, normally found throughout body, has been used as abiological material for correction of various tissue defects in plastic, cosmetic and reconstructivesurgery fields. Lipoaspiration or resection of adipose tissue and fat grafting are routinely carried outin clinic with minimal risk. Adipose tissue is well-vascularized, and fat cells have a relatively longlifespan. Adipose tissue is now recognized as an important endocrine and secretory organ of variousbioactive cytokines to regulate homeostasis of human body. The accumulated evidence attractsmuch attention in regenerative medicine as cell source for gene/cell therapy. There are two methodsin the preparation of adipose tissue-derived proliferative cells. One method is preparation fromstromal vascular fraction, which is obtained as sediment by the centrifugation of collagenasedigestedfat tissue and is the most common technique. The other is from the floating fraction afterthe centrifugation to obtain mature fat cells. The cells are further purified by ceiling culture. In thisreview, we introduce the outline of preparation of primary adipocytes and our therapeutic approachof an adipose tissue-based lifelong and risk-manageable treatment for patients with intractableserum protein deficiencies.
