Towards the generation of human iPSC-derived hematopoietic stem cells

Abstract

Generation of hematopoietic stem cells (HSCs) from embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs) has been a long-standing, challenging goal of hematology. In this review, current advances toward the goal utilizing various in-vitro differentiation techniques and animal in-vivo microenvironments will be summarized. In addition, we will introduce our unique method of sheep in-utero transplantation targeting a fetal liver as an in vivo microenvironment for hematopoietic engraftment.