Abstract
Chondroitin sulfate of acid mucopolysaccharides is known to play an important role mineralization.
We have been interested in the development of temporal bone because the otic capsule takes unique ossification course and the physiological characters in the cartilage bone.
The animals used in this experiment were ranging in age from the 18 th day prenatal to the 5 th day postnatal. Basic procedure in autoradiograical study was the stripping-film technique described by S.R.Pelc. Animals were injected intraperitoneally with 35S and the otic capsule was collected one hour after injection. Serial sections placed by stripping film were stored in light-tight boxes for exposure and stained after processing procedure.
35S activity in microscopic autoradiogram is expressed number of black dots exposed from radioisotope.
Regional differeces of the 35S activity in the otic capsule of 18 th day-prenatal-rats were observed. High 35S activity is regarded as concentration of acid mucopolysaccharides.
We presume that the vectors correspond with growing direction. 35S activity is coincident with direction of vectors and weakens in the areas grown rapidly.
