ANALYSIS OF PERIPHERAL T LYMPHOCYTE SUBSETS IN PATIENTS WITH HEAD AND NECK MALIGNANT TUMORS

Two COLOR ANALYSIS USING BY FACS

Abstract

Peripheral blood lymphocytes (PBL) on T lymphocyte subsets in 20 patients with head and neck malignant tumors and in 20 healthy controls were determined in Fluorescence Activated Cell Sorter (FACS) using fluorescein isothiocyanate-conjugated monoclonal antibodies (anti-Leu 2 a, and-Leu 3 a) and phycoerythrinconjgated monoclonal antibobies (anti-Leu 8, anti-Leu 15).
The results obtained were as follow:
1. Leu 3 a⁺Leu 8^-cells in patients were significantly increased than healty controls.
2. Leu 2 a⁺Leu 15⁺ cells showed no differance between patients and healthy controls.
3. Leu 3 a+ Leu 8^-/Leu 2 a⁺Leu 15⁺ratio was significantly increased in patients than in healty controls.