Three dimensional structure of the vestibular sensory epithelia

Abstract

Vestibular neurosensory epithelia of the guinea pig and the bull frog were investigated by scanning electron microscope. The crista ampullaris or macula were freezecracked and maceration with 0.1% OsO4 solution was added for 24-60 hr. (Osmium-DMSO-Osmium method). Following this method, three dimensional intracellular structures were observed. The mitochondria which exist in the nerve chalice surrounding the type I cell were various, globular, long and slender. Golgi apparatus, endoplasmic reticulum and thin fibrous structures of the sensory cells or supporting cells were clearly demonstrated. Concerning nervous system, afferent and efferent nerve endings, furthermore synaptic structures were also observed stereoscopically.