Synthesis of 5-, and 15-HETE in the Experimental Otitis Media
1984 Volume 15 Issue 1 Pages 40-42
Details
1984 Volume 15 Issue 1 Pages 40-42
In order to clarify the pathogenesis of otitis media, we used guinea pig as a model for studying the effect of inoculation of Haemophilus influenzae into the middle ear cavity. Histological changes and variations of 5- and 15-HETE synthesis in the guinea pig middle ear mucosa were investigated.
5- and 15-HETE is producted when arachidonic acid of plasma membrane converted to SRS-A which is thought to be a chemical mediator of inflammation.
(1) Formalin-inactivated H. influenzae induced inflammation in the middle ear mucosa, but not so strong as non treated H. influenzae.
(2) Synthesis of 5-HETE in the non treated H. influenzae group increased from 1 to 3th day, but decreased from 5 to 7th day. Though in the formalin-inactivated group decreased from 1 to 7th day.
(3) Synthesis of 15-HETE in the both group increased from 1 to 3th day, then decreased 5 to 7th day.
