Histopathological study in the endolymphatic sac

The number of macrophages

Abstract

We studied the histopathological changes in the endolymphatic sac. Thirty one Hartley guinea pigs were immunized with stria vascularis of rabbits in Freund's complete adjuvant, and 13 guinea pigs were inoculated with analphylatoxin via the carotid artery. Twelve guinea pigs with a normal Preyer's reflex, served as the control group. The temporal bones were fixed in 10% buffered formalin, embedded in celloidin, cut into section of 13μ and stained with hematoxylin and eosin.
We observed and counted macrophages in the whole endolymphatic sac. We could not find any histopathological changes, but there were more macrophages in the endolymphatic sac, in the immunized and inoculated animals than in the control ones. The results suggests that the endolymphatic sac is involved in the immune response.