1989 Volume 20 Issue 1 Pages 365-366
DF-100 (Puri Dyne, Inc., N. C., USA) is a natural substance extracted from seeds of grapefruits, which has been reported to have antimicrobial activities such as antifungal, antibacterial and antiviral activities. DF-100 has been thought to have a possibility of usefulness in cases of various infections because of its low irritability to tissues. In this paper, DF-100 was tested for the toxicity on the mucosa of the eustachian tube of guinea pigs. DF-100 was also tested for its antimicrobial activity by measuring its MIC against Candida, S. aureus (MRSA) and Pseudomonas sp. DF-100 induced significant ciliary inhibition at concentrations higher than 500ppm, whereas 5Oppm of DF-100 induced mild ciliary inhibition, which recovered following irrigation with Krebs Henseleit buffer. MIC of DF-100 against Candida sp. was less than 100ppm. Its MICs against Pseudomonas aeruginosa and Ps. cepacia were more than 3, 600ppm whereas 3.15ppm against S. aureus (MRSA).
