Tissue Culture of Middle Ear Epithelium of the Guinea Pig

Topographical Differences of the Cellular Growth Activity in the Mid-dle Ear Cavity

Abstract

Explants from middle ear and tracheal mucosa of the guinea pig were cultured using collage gel method, and their proliferative characteristics were studied. The outgrowthal area of cultured cells was examined quantitatively and compared at each location for periods of up to ten days. The specimens sampled from the sites with cuboidal half-ciliated epithelia, which formed as large as tenfold out-growthal sheets from the initial explant, showed higher proliferative ability than the sites with pseudostratified ciliated epithelia.