Abstract
To clarify the transport mechanism of organic anions into the endolymph, we investigated the concentrations of furosemide (FU), one of the organic anions, in the endolymph taken from the scala media of the guinea pig cochleae. Less than 300 nl of the endolymph were taken with the double barreled pipette monitoring EP after intravenous administration of 100mg/kg FU. The concentrations of FU were measured by HPLC using the fluorescence detector. FU concentrations in the endolymph reached the maximum value within 60 minutes and gradually decreased thereafter. This elimination pattern in the endolymph is pronouncedly different from that in the perilymph we have reported previously.
