Inflammatory Process due to Escaping Debris from the Cholesteatoma

Abstract

The keratin granulomas were observed not infrequently in the surgical specimen of a bone destructive cholesteatoma, however, it is not clearly demonstrated as to how the escaping debris induce an inflamatory process or how the keratin, one of the contents of debris, works in this process. In order to observe this process, we inplanted the porous subcutaneous chambers, which contain debris of a human cholesteatoma, LPS or gelatine, and observed histologically or immunocytochemically using RAMll after 1, 2 or 3 weeks, respectively. Highly inflammatory cell ingrowth were observed in the chember of debris 1 week after inplant. Although the cell infiltration and necrosis progressed in the debris chember after 2 and 3 weeks passed, the keratin were still observed. The keratin seems to work in prolonged or repeated inflammatory process.