Nonlinear optical imaging using a supercontinuum light source

Abstract

Supercontinuum light sources are powerful light sources for spectroscopic imaging. Using this laser source, we developed a multi-modal (coherent anti-Stokes Raman scattering (CARS), second harmonic generation (SHG), sum-frequency generation (SFG), third harmonic generation (THG), third-order sum frequency generation (TSFG)) nonlinear optical imaging system, and visualized living cells and tissues without staining or molecular labeling.