Analysis of tritium-induced cancer using a highly sensitive mouse model for carcinogenesis

Abstract

We aim to evaluate the carcinogenic risk of low dose rate exposure to tritium using carcinogenic hypersensitive model mouse (Apc^{Min / +} mouse) and to clarify its carcinogenic mechanism. Tritiated water of 90 MBq was intraperitoneally injected to the Apc^{Min / +} mice (F1) which mice generated from C57BL / 6J-Apc^{Min / +} mice (♂) and C3H mice (wild type, ♀) at 2 weeks of age. The mice were kept for 25 weeks and the number of small intestine tumors were measured. As a result, mice injected tritiated water showed significant differences in the increase in the number of small intestine tumors, the increase in spleen weight and the decrease in hemoglobin levels. Currently, DNA is purified from cells taken from a part of a tumor, and LOH analysis is performed on chromosome 18 using a Mit marker.