Abstract
Sonolytical inactivation of Cryptosporidium oosyst at frequencies of 28, 40, and 100kHz has been studied. At 28kHz oosysts had breaks in their wall and nuclei had leaked. More than 99.9% of oosysts were inactivated after 10 min sonication with 28kHz, however only 10% with 40kHz and no inactivation with 100kHz. Mice orally took the sonolized oosysts excreted no cryptosporidium oosysts even after two weeks.