Abstract
The Kokubo′s simulated body fluid (SBF) has been extensively used for evaluating their in vitro apatite-forming ability and potential as bioactive materials. The present study examined the effects of organic acids and albumin in the SBF on apatite formation on glass and titanium substrates. Titanium substrates were subject to a H2O2 treatment subsequent heating to prepare titania layers. Apatite formation was then examined after soaking the substrates in both SBF and modified SBF containing organic components such as succinic acid, tartaric acid, citric acid and albumin. Calcium-chelate formation due to the organic acids reduced free calcium ion concentration. The induction time of apatite nucleation on the titania surface was delayed in SBF containing the organic acids. The reduction in the free calcium ion concentration suppressed the apatite nucleation.
