Cell Activation on Biocomposite Scaffold Formed on Biomaterials

Abstract

The deposited layer composed by a biological apatite and on adsorbed serum protein was termed ″biocomposite scaffold″. The ceramic HAp and titanium plate were used as the substrate of the forming biocomposite scaffold by soaking in culture media. The biocomposite scaffold was formed on the ceramic HAp and the surface-modified titanium. Cell cultivation test using various type cells indicated that a number of adhering cells was observed on the biocomposite scaffold. Cell adherence on the biocomposite scaffold was reinforced in particularly osteoblast-like cell. It was suggested that the included serum protein played a great role of the cell activation, and MC3T3-E1 cell with a phagocytosis induced a closed contact. Consequently, the cell activation was induced only by a concerted reaction of biological apatite formation and serum protein adsorption.