Effects of organic molecules in modified simulated body fluid on apatie formation on glass and titanium substrates

Abstract

Titanium substrates were subject to a H₂O₂ treatment subsequent heating to prepare titania layers. Apatite formation was then examined after soaking the substrates in both Kokubo's simulated body fluids (SBF) and modified SBF containing organic components such as succinic acid, tartaric acid and citric acid. Calcium-chelate formation due to the organic acids reduced free calcium ion concentration. The induction time of apatite nucleation on the titania surface was delayed in SBF containing the organic acids. The reduction in the free calcium ion concentration suppressed the apatite nucleation.