Design and Estimation of buffer solution for DNA separation by using Ceramic nano-particles

Abstract

In this work, we design the buffer solution structures by addingmonodispersed ceramic nanoparticles to the buffer solution. This workfocuses the effect of the buffer solution structures on DNA analysis bymicro-capillary electrophoresis, which will realize high-mobilityand high-resolution of double-strand deoxyribonucleic acid (dsDNA) analysis.Various particle sizes of monodispersed SiO2 and other nanohybridnanoparticles were mixed with conventional 0.7% hydroxyl propyl methylcellulose (HPMC) buffer solution to design the buffer solution structures.These buffuer solution design achieved perfect separation of the DNAspecimen (100 bp to 1.5 kbp) if buffer solution designs were optimized. Wealso discuss the mechanism of the improvement for DNA analysis by usingceramic nanoparticles during electrophoresis.