Abstract
A laminin-immobilized apatite layer and an apatite layer were formed on the surface of a substrate of ethylene-vinyl alcohol copolymer (EVOH). Share strength of the laminin-immobilized apatite layer was higher than that of the apatite layer under the wet condition. After these specimens were dried, however, share strength of the laminin-immobilized apatite layer became lower than that of the apatite layer. This might be due to a conformational change of the laminin molecules in the laminin-immobilized apatite layer during the drying process. Adhesive strength of the laminin-immobilized apatite layer to the EVOH substrate was lower than that of the apatite layer under a dry condition. In conclusion, a laminin-immobilized apatite-EVOH composite prepared by the present process should be used for percutaneous devices without being dried after the preparation.
