Abstract
A variety of nitrogen-containing drugs used to treat heart disease were separated on ZORBAX Rapid Resolution (RR), 3.5 μm, and Rapid Resolution High throughput (RRHT), 1.8 μm, columns of differing dimensions. All three column configurations produce satisfactory results, creating the luxury of deciding which column configuration to choose for finalizing a method. Speed, resolution and sensitivity are primary goals in method development and column selection. When using RR or RRHT technology, these three factors can be quickly achieved using more than one column configuration. In this application we can compare three RR and RRHT columns, considering speed, sensitivity, and resolution to make a column choice. Additionally, with the primary goals met, we demonstrate how high temperature can be used to alter selectivity and further improve resolution of critical pairs in the chromatogram.
