Abstract
We studied the effect of Platelet-Rich Plasma alone (PRP) weekly compared to flap operation (FOP) using an enamel matrix derivative (EMD), after experimental periodontal breakdown was induced around the 2nd premolar (P2) and 3rd premolar (P3) of the lower and right left jaw in dogs. Sbjects were 6 beagles. Bilaterally mucoperiosteal flaps were raised. Single-wall osseous defects were created on the mesial aspect of P2 and distal aspect of P3 and at insertion forced down toward periodontal pockets. Beagles under went periodontal surgery 3 weeks after defects were created; this time was made the baseline. Beagles were divied into 3 groups—a FOP group for FOP alone, a PRP group applying PRP, and an EMD group applying EMD (Emdogain® Gel). FOP was companed to PRP on P2 and EMD to PRP on P3. Periodontal parameters, i.e., probing pocket depth (PPD), probing attachment level (PAL), tooth mobility (TM), gingival crevicular fluid volume (GCF), and X-ray assessment were measured at baseline 6, 8, 10, and 12 weeks after periodontal surgery. From 6 weeks, daily plaque was controlled by tooth cleaning 3 times a week. After the experiment, histological sections were processed and analyzed.
Results were as follows : In P2, TM and GCF improved more in the PRP group than in the FOP group (p<0.05). In P3, no significant difference was seen in periodontal parameters between the EMD and PRP groups, although EMD showed a marked gain in forming new cementum compared to PRP (p<0.05).
Our results suggest that PRP alone heals wounds earlier than FOP for single-wall osseous defects, while PRP had no difference compared to EMD at the clinical level, and PRP alone did not necessarily effectively improve PPD and PAL in periodontal tissue with single-wall osseous defects.
