Abstract
We studied the possibility of using grafts composed of human alveolar bone periosteal cells (HABPCs) for periodontal regeneration. HABPCs were inoculated four times to become a multilayer cell sheet every week that was cultured for three days after buing folded to create the graft. Graft morphology and protein expression were analyzed using histological (hematoxylin and eosin, alkaline phosphatase (ALP) and von Kossa′s staining) and immunohistochemical (type I collagen (Col-I) , osteopontin (OPN) , osteocalcin (OC) and Ki-67) studies on graft sections.
The graft must be able to be used with tweezers easily, must be about 5×10 mm in size, and must b about 1.5 mm thick. As a result of histological observation, the graft consisted only of the cell and the extracellular matrix. Immunohistochemical staining of graft sections showed a strong positive reaction for Col-I and included OC, OPN, and Ki-67 positive cell.
Grafts may thus be calcified by the above results. Nihon Shishubyo Gakkai Kaishi (J Jpn Soc Periodontol) 48 : 192-200, 2006.
