Periodontal regeneration by BMP-2 solution and collagen hydrogel application to root dentin surface.

Abstract

We evaluates periodontal regeneration in a dehiscence model after the application of BMP-2 solution and collagen hydrogel to the root dentin surface.
Materials were 128 premolars of 8 beagles. After a partial thickness muco-gingival flap on the buccal site was elevated, periodontal dehiscence defects with a length of 6 mm were surgically produced. Root surfaces were scaled and planed to remove periodontal ligament and cementum.
Denuded dentin surfaces were subsequently demineralized with 24% EDTA and divided into four groups. In the control group, neither BMP-2 solution nor collagen hydrogel was applied. In the Gel group, collagen hydrogel was applied to root dentin surfaces. In the BMP group, BMP-2 (1000 μg/ml) solution was applied to root dentin surfaces. In the BMP-Gel group, BMP-2 (1000 μg/ml) solution and collagen hydrogel were applied to root dentin surfaces. Histological analysis was conducted 1, 2, 4 and 8 weeks after surgery. Histometrical analysis was conducted 4 and 8 weeks after surgery.
At 8 weeks, new bone formation in the BMP-Gel group was significantly greater than in any other group. New cementum formation in the BMP-Gel group was significantly greater than in the control group. Ankylosis in the BMP-Gel group was significantly greater than in the control group. Down-growth of the junctional epithelium in the BMP-Gel group was significantly smaller than in the control group.
These results suggest that the application of BMP-2 solution and collagen hydrogel to root dentin surfaces is conducive to periodontal tissue regeneration. Nihon Shishubyo Gakkai Kaishi (J Jpn Soc Periodontol) 48 : 255-266, 2006.