Abstract
This study evaluated periodontal regeneration after the application of BMP-2 to root dentin surface in 1-wall infrabony defects.
The mandibular first and third premolars in seven beagle dogs were extracted prior to experimental surgery. Eight weeks after the extraction, the periosteum was removed from the alveolar bone following the retraction of a partial thickness of the muco-gingival flap. Subsequently, 1-wall infrabony defects (5 mm in height, 3 mm in width) were surgically created in the mesial and distal sides of the mandibular second premolars, and in the mesial sides of the mandibular fourth premolars. Then, the periodontal ligament and the cementum on the exposed root surface were thoroughly removed. The denuded root surface was demineralized with 24% EDTA for 3 minutes. In the BMP group, BMP-2 solution (1000 μg/m) was applied to the root surface, and the flap was repositioned. BMP-2 was not applied in the control group. Histological and histometrical analyses were performed at four and eight weeks after surgery.
At eight weeks,new bone formation, new cementum formation and ankylosis were significantly greater in the BMP group than in the control group (p<0.01). The down-growth of the junctional epithelium in the BMP group was significantly smaller than that in the control group (p<0.05).
These findings suggest that periodontal regeneration was enhanced by the application of BMP-2 to the root dentin surface in 1-wall infrabony defects.
Nihon Shishubyo Gakkai Kaishi (J Jpn Periodontol) 49 : 296-304, 2007.
