1975 Volume 17 Issue 1 Pages 59-66
The histamine contents in whole saliva and gingival exudate collected from patients with periodontal disease were measured by both Namiki's modified and Anton-Sayre's method. Comparative study of both methods were performed to find out the better way of estimating histamine.
The following results were obtained:
1. Although Namiki's modified method was able to count out the range from 1 to 60μg/ml of histamine, Anton-Sayre's method was from 0.05 to 10.0μg/ml. Therefore, the later was much superior than the former for analysis of very small amounts of histamine.
2. Namiki's modified method was capable of measuring of histamine contents in whole saliva of all subjects, but of only 5 in 11 subjects in case of gingival exudate. However, Anton-Sayre's method was able to measure histamine contents of all subjects both in whole saliva and gingival exudate.
3. The histamine contents in whole saliva and gingival exudate were distinctly high in patients with periodontal disease.
