Abstract
The purpose of this paper is to detect antibodies to collagen in sera of patients with periodontal disease by passive haemagglutination,
Human gingival acid soluble collagen and bovine skin acid soluble collagen were used as antigens.
Determination of anticollagen antibody titers in human sera was carried out on 10 clinical healthy subjects ranging from 23 to 27 years of age and in 57 patients with periodontal disease ranging from 16 to 69 years of age. The correlation between anti-collagen antibody titers and a few indices of periodontal disease was discussed to estimate antibody titers as an index of periodontal tissue destruction.
The following results were obtained.
1. Human gingival acid soluble collagen was found to have antigenicity.
2. Bovine skin acid soluble collagen was found to have antigenicity, too. And antiserum to bovine collagen had cross reaction to human gingival collagen.
3. Antibodies to human gingival collagen were not detected in any sera of 10 clinical healthy subjects, but in 6 sera of 57 patients. But no significant correlation was found between antibody titers and clinical indices, i.e. Gingival-Bone count, salivary leucocyte count and total hydroxyproline levels in sera.
4. Antibodies to bovine skin collagen were detected in 37 sera of 57 patients and in 4 sera of 10 clinical healthy subjects. But no significant correlation was found between antibody titers and clinical indices, i.e. Gingival-Bone count, salivary leucocyte count and total hydroxyproline levels in sera.
